ABSTRACT

It has become more crucial to use fluorescence in situ hybridization (FISH) as a diagnostic and monitoring technique for the detection of disease-related chromosomal aberrations. Interphase FISH (iFISH) analysis was performed on 92 individuals. For the detection of common cytogenetic rearrangements linked with haematological malignancies, we have employed five distinct FISH probes. Patients with BCR/ABL gene rearrangements were tested in a total of 83 cases. 37.3 percent of patients (31/83) had iFISH patterns of BCR/ABL gene rearrangements that varied from 10 percent to 98 percent. While three individuals with AML had t (15; 17) (12%), and inv (16; 16) (8.3%), t (8; 21) was absent in the study. There were 6.5 percent of all instances in this investigation where secondary chromosomal abnormalities were found to be non-random. Patients with CML who have BCR/ABL gene rearrangements are likely to benefit from using this information to monitor their treatment. In addition, atypical patterns may have clinical consequences. The function of AML1/ETO, PML/RARA, CBFB and p53, as well as the particular chromosomal locations and interacting genes, must be studied in bigger groups of patients.

KEYWORDS: – Fluorescence in Situ Hybridization, BCR/ABL, Gene Rearrangements